Analysis Note

ControlHuman lung tissue lysate

Application

Research CategorySignaling

This Anti-Surfactant Protein B Antibody is validated for use in WB, IH(P) for the detection of Surfactant Protein B.

Immunohistochemistry Analysis: 1:200 dilution from a representative lot detected Surfactant protein B in clara cells in normal rat lung tissue.

Research Sub CategoryLipid Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Surfactant Protein B (SP-B) is mostly composed of phospholipids, but also incorporates essential proteins. It is necessary for pulmonary surfactant function and the alveolar type 2 cell phenotype. SP-B deficiency has been known to cause congenital pulmonary alveolar proteinosis. SP-B is thought to protect lungs against endotoxin-induced inflammation. SP-B could be used as a clinical marker in chronic heart failure.

Immunogen

Epitope: Saposin B-type 2 domain

GST-tagged recombinant protein corresponding to human Surfactant Protein B at the Saposin B-type 2 domain.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: 1:5,000 dilution of this antibody detected Surfactant Protein B on 10 µg of human lung tissue lysate.

Specificity

This antibody recognizes Surfactant Protein B at the Saposin B-type 2 domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~16 kDa observed